Polyhydroxyalkanoates from pear (Pyrus communis L) waste by Bacillus subtilis

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Cynthia Estefania Hernández-Manjarrez
Alejandro De Jesús Cortés-Sánchez
Miriam F. Fabela-Morón
Adolfo A. Rayas-Amor
Mayra Díaz-Ramírez

Keywords

Bacillus, pear, biodegradable plastic, polyhydroxyalkanoates.

Resumen

Objective: Analyze the production of polyhydroxyalkanoates (PHA) by Bacillus subtilis sp., subtilis in submerged culture using pear residues as a carbon source.


Design/methodology/approach: The culture consisted of pear residue flour concentrations of 5 and 15 % for 72 h. Reducing sugars, biomass, pH, protein, and pHA extraction and quantification were analyzed during submerging cultivation of Bacillus subtilis sp., subtilis


Results: The results showed that Bacillus subtilis sp., subtilis can grow and generate PHA, having its maximum proportion in PHA extract of 0.094 g/L at 72h of culture, at a substrate concentration of 5%.


Study limitations/implications: More studies related to the optimization of culture conditions for PHA production are required and prior treatment of pear waste flour will be considered.


Findings/Conclusions: The synthesis of these biopolymers is important to promote their large-scale production using agro-industrial waste, thus contributing to reduce the environmental impact.

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