PHYTOSANITARY CERTIFICATION OF VITROPLANTS OF Saccharum spp., TO ESTABLISH BASIC SEEDBEDS

Abstract

The in vitro sanitation and propagation of sugar cane (Saccharum spp.) is important to obtain certified seedbeds for plants that are free of pathogens, genetically homogeneous and invigorated. The process of certification of sugar cane seedling production is described, before the National Service for Agrifood Sanitation, Innocuousness and Quality (Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria, SENASICA), using Plant Tissue Cultivation (PTC) for the establishment of basic and certified seedbeds. Apices were used as explants subjected to thermos-hydrotherapy and were cultivated in vitro in semisolid Murashige and Skoog medium, with growth regulators, to induce cellular regeneration, and silver nanoparticles were applied to eliminate diseases. After three sub-cultivations, buds were taken as samples of plant material and sent to the National Center for Phytosanitary Reference (Centro Nacional de Referencia Fitosanitaria, CNRF) for their diagnosis, and it was negative for the principal sugar cane diseases. Finally, the buds were transferred to Temporary Immersion Bioreactors for their large-scale multiplication to establish basic and certified seedbeds