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The objective was to determine the effect of Azospirillum sp. microorganisms (biofertilizers), and arbuscular mycorrhizae (Glomus sp.) on the growth of Brachiaria brizantha. The study was performed in a completely random design with 2x6x2 factorial arrangement (two cultivars, six methods of fertilization, and two forms of water supply), evaluating the percentage and index of germination speed, height, dry matter yield, root:aerial part rate. With regard to the “A” factor, the mulato II cultivar produced 6.19 t ha-1 DM 44 days after sowing (DAS). The “B” factor presented high yields in plants with chemical fertilizer and mycorrhizae combination with Azospirillum, showing up to 50.83 % of germination, 4.07 plants at 44 das, and higher leaf production yield with combined strains of mycorrhizae with Azospirillum. The “C” factor affected the yield of the plants with water stress. The combined fertilization mycorrhizae-Azospirillum increased the yield of fodders.
