Germination test in NaCl solution on corn (Zea mays L.) seeds

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Abel Santillán Ángeles
Abel Quevedo-Nolasco
Apolinar Mejía-Contreras
Francisco Gavi- Reyes†

Keywords

Hibrid, saline solution, initial seed vigor, physiological quality

Abstract

ABSTRACT


Objective. To evaluate germination behavior and define initial vigor in maize seeds.


Methodology. The genetic material used were four batches of 20 hybrid seeds (M-2014, SR-2014, SR-2012 and SR-2011) of each one, which were germinated in a NaCl solution (0, 5, 10, and 15 dS m-1) in a germination chamber. A Randomized Complete Block experimental design was used, where the experimental unit was the seed lot and salinity level; with four replicates each. Once seeds with defined radicle and plumule were observed, they were counted for evaluation.


Results. When comparing the three lots from the same environment (production site) and different year, the statistical analysis indicated that longer seeds had lower germination percentages. Similarly, when comparing the lots from different production environments and the same year, M-2014 obtained the highest average germination percentage (92.19%). When evaluating the maximum salinity level, 15 dS m-1, a loss in the average germination percentage of 41.25% was observed.


Conclusions. The test was acceptable to evaluate the viability and vigor of a seed lot, because it was efficient in discriminating seed lots in relation to speed and uniformity in germination based on the NaCl solution.


Key words: Hybrid, saline solution, initial seed vigor, physiological quality.

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