Objective: To isolate and determine in a greenhouse environment the biofertilizing
potential of rhizospheric bacteria associated to corn (Zea mays L.) at Campeche,
Design / methodology / approach: Rhizospheric soils were collected from two
corn production zones with different management conditions. Bacterial strains were
isolated from these samples and their biofertilizing potential determined by in vitro2 and in vivo tests. The obtained data from both tests were assessed using an
analysis of variance (ANOVA) and a means comparison test (LSD, p ? 0.01).
Results: In total, 16 rhizospheric bacteria were isolated, a higher number in non-
mechanized soils (n = 10) compared to mechanized ones (n = 6). In the in vitro
tests, the most representative activity corresponds to nitrogen fixation (81%)
attributed to a higher bacteria percentage, while the activity with lower bacteria
numbers corresponds to IAA production (25%). At the in vivo tests in corn plants,
the YM1 strain presented the highest fresh and dry root biomass (20 and 2 g plant -
1 , respectively). The YM4 strain promoted greater plant height (63.33 cm), and YM5
registered the highest values in stem diameter (7.13 mm), root length (36.78 cm)
and fresh shoot weight (12.03 g plant -1 ).
Limitations / Implications: Strain evaluations were limited to controlled
Conclusion: The YM1, YM4 and YM5 strains show potential for further evaluation
as biofertilizers for corn cultivation under field conditions.